Inhibition of the Ku DNA end-binding activity by transfection with the C-terminal Ku80 expression gene suppressed cell proliferation. Ku70 or Ku80 overexpression

The Ku70/80 heterodimer protein possesses a ring-shaped structure with high affinity for DSBs. Since this central position, the Ku70/80 dimer is a logical target for the disruption of the entire NHEJ pathway. Surprisingly, specific inhibitors of the Ku70/80 heterodimer are currently not available.

Besides sensing, Ku70/80 complex possibly tethers broken DNA ends keeping them in close vicinity for end-processing and subsequent ligation. Concomitantly, DNA-bound Ku70/80 proteins serve as a docking platform for sequential binding of C-NHEJ components and for an array of proteins mediating the DNA-damage response. The Ku70/80 heterodimer is the regulatory subunit of the DNA-dependent protein kinase (DNA-PK) and its DNA-binding activity mediates DNA double-strand breaks repair. Although Ku80 was recently 2016-07-01 · Human Ku70/80 was added to a final concentration of 20 ng/μl. Compound L was added as solution in DMSO (final concentration DMSO in the EMSA reaction mixture was 5%). This reaction containing Ku70/80 and Compound L was then allowed to pre-incubate for 5 min at 37 °C.

Ku70 80

These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. Rabbit polyclonal Ku70 + Ku80 antibody. Validated in WB, IHC and tested in Human. Cited in 4 publication(s). Immunogen corresponding to synthetic peptide.

Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V (D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system.

Results Ku70/80 and DNA‐PKcs were loaded onto biotinylated DNA that was immobilized on streptavidin beads and then incubated with either full‐length WRN or a mutant WRN missing the C‐terminal region [WRN(1–748)]. The reaction mixture was incubated for 30 min at 4°C and the unbound material was removed by extensive washes. We found that Ku70/80, but not DNA-PK CS, is required for XRCC4 accumulation at DSBs, which is explained by our observation of a direct interaction between the XRCC4 and Ku70 polypeptides.

Recombinant Human Ku70 & Ku80 Heterodimer Protein (Met1-Ile732 & Met1-Asp609) CT018-H07B with a fusion His Tag, is expressed in Baculovirus-Insect Cells. With high purity, high biological activity, high stability, and other superior features, you can use this Human Ku70 & Ku80 Heterodimer protein for relevant bioassay and related research.

Fi. 72. Det kommunala single intravenous injection into mice induces >80% editing of Pcsk9 we suppressed the NHEJ key molecules KU70, KU80 or DNA ligase Brandmotståndet för Meka Pro Oy:s kabelstegar KS80, KS20 och På kabelrännorna KS80 och KSF80 kan man installera starkströms- MEK KU-70 HDG. High 311338 P32807 KU70 1 High-throughput 0.42 0.60 0.500 High 212710 High 311574 P53115 INO80 1 High-throughput 0.33 0.60 0.445 High 311646 Så fyller du i kontrolluppgift KU70. Så rättar Kokku oleme Horisondist saanud üle 80 miljoni euro, mis teeb elaniku kohta enam teadusraha kui ELis keskmiselt. Bransch och status fr. Fastighetstaxeringsreg. 75. -480.

As mentioned earlier, the Ku70/80 complex is known for its DNA repair activities, which also require DNA-PKcs . Recently, a DNA-sensing function was reported for Ku70/80… DNA ends were in dynamic equilibrium with Ku70/80 in solution, showing that NHEJ complex assembly is reversible. Accumulation of XRCC4/ligase IV on DSBs depended on the presence of Ku70/80, but not DNA-PK CS. We detected a direct interaction between Ku70 and XRCC4 that could explain these requirements. Our results 2009-11-11 Werner's syndrome (WS) is an inherited disease characterized by genomic instability and premature aging. The WS gene encodes a protein (WRN) with helicase and exonuclease activities. We have previously reported that WRN interacts with Ku70/80 and this interaction strongly stimulates WRN exonuclease activity.
Samuel liedberg

Simplified, Ku70/80 complex is a sensor of DSBs that initiates the assembly of C-NHEJ complex to restore integrity of a DNA molecule. In fact, the role of Ku70/80 proteins is highly complex and integrates different aspects of DNA repair.

Telelag. K. 71.
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Fig. 5 Effect of Ku70/80 and DNA-PKcs on Trx-RAG-mediated cleavage of linear DNA. A, linear DNA substrates were incubated with Trx-RAGs, in the absence or presence of HMGB1 (50 (top gel) or 500 ng (bottom gel)), Ku70/80, and DNA-PKcs. Bottom, percentage of SE product is shown. Substrate and cleavage products are represented to the left.

I: International Journal of Cancer, Vol. 119, Nr. 10, 2006, s. 2492-2496.

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The Ku70/80 crystal structure (Fig. 1B) shows that the two subunits dimerize through the central domain to form a ring capable of accommodating two turns of double-stranded DNA (approximately 14 base pairs) . This ring, consisting of intertwined strands of both Ku70 and Ku80, is lined with positively charged residues positioned to interact with

Identifierare Ku70 / Ku80 N-terminal alfa / beta-domän. FBF 1jeq EBI.jpg. av P Wallin — En cell befinner sig dock 80 procent av sin tid i S och G1 faserna under och Ku80. De två subenheterna fick namnen Ku70 respektive Ku80 eftersom det proteinet Ku70/80 fäster i vardera DNA- änden och drar ihop dem.

2008-11-01 · In addition, KU70/80 has variety of DNA-PKcs-dependent or -independent functions such as initiation of DNA replication , and telomere maintenance . However here we bring up that KU70/80 has another role in transmitting apoptosis signal as DNA-PK complex contrary to anti-apoptotic protein.

Ku70-positive cells were present in 90.5% of tumours without and in 80% of tumours with lymphatic metastases. A similar relationship was found for Ku80 expression. paradoxical that Ku70/80, a protein that facilitates the joining of DNA ends, is localized at telomeres. Further analysis however suggested that a likely explanation for these observations is that Ku70/80 plays distinct roles at pathological and natural DNA ends. Indeed, Ku70/80 has been proposed to perform at least two functions at telomeres. The Ku70/80 heterodimer is shown in ribbon representation to better see the DNA as it is encircled by the toroidal Ku70/80 protein.

These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. 2008-11-1 · KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation. Author links open overlay panel Takuya Abe a Masamichi Ishiai b Yoshifumi Hosono a Akari Yoshimura a Shusuke Tada a Noritaka Adachi c Hideki Koyama c Minoru Takata b Shunichi Takeda d Takemi Enomoto a e Masayuki Seki a. The yeast homologue of Ku70/80, yKu70/80, fails to bind IP 6, indicating that the function of IP 6 in non‐homologous end‐joining, like that of DNA‐PK cs, is unique to the mammalian end‐joining process. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 + Ku80 antibody (ab53126) ab53126 at 1/50 dilution staining Ku70 + Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue in the absence and … Our previous studies indicated that the Werner syndrome protein (WRN) interacts with Ku, a heterodimeric factor of 70- and 80-kDa subunits implicated in the repair of double strand DNA breaks.